COVID-19: variation analysis on ARTIC PE data

public public 1yr ago Version: v0.5 0 bookmarks

COVID-19: variation analysis on ARTIC PE data

The workflow for Illumina-sequenced ampliconic data builds on the RNASeq workflow for paired-end data using the same steps for mapping and variant calling, but adds extra logic for trimming amplicon primer sequences off reads with the ivar package. In addition, this workflow uses ivar also to identify amplicons affected by primer-binding site mutations and, if possible, excludes reads derived from such "tainted" amplicons when calculating allele-frequencies of other variants.

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Created: 1yr ago
Updated: 1yr ago
Maitainers: public
URL: https://github.com/iwc-workflows/sars-cov-2-pe-illumina-artic-variant-calling
Name: sars-cov-2-pe-illumina-artic-variant-calling-covid
Version: v0.5
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Copyright: Public Domain
License: MIT License
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