wrapper:
    "v1.7.1/bio/fastqc"

shell:
    "bowtie2 -p 8 --local --no-discordant --no-mixed "
    "-x {params.bowtieindex}"
    "-1 {input.r1} "
    "-2 {input.r2} "
    "-S {output} "

shell:
    "samtools view -bS {input} > {output} "

shell:
    "plotPCA --corData {input.countnpz} "
    "--plotFile ./results/MultiBamSummary/deepTools_pcaplot.png "
    "-T 'PCA of read counts' "
    "--outFileNameData ./results/MultiBamSummary/deeptools_pcaProfile.tab "

shell:
    "macs2 callpeak -t {input} -n {wildcards.sample} --outdir results/MACS2/{wildcards.sample} --nomodel --shift -100 --extsize 200 -g mm "

__author__ = "Johannes Köster, Christopher Schröder"
__copyright__ = "Copyright 2016, Johannes Köster"
__email__ = "[email protected]"
__license__ = "MIT"


import tempfile
from snakemake.shell import shell
from snakemake_wrapper_utils.java import get_java_opts

log = snakemake.log_fmt_shell()

extra = snakemake.params.get("extra", "")
java_opts = get_java_opts(snakemake)

bams = snakemake.input.bams
if isinstance(bams, str):
    bams = [bams]
bams = list(map("--INPUT {}".format, bams))

if snakemake.output.bam.endswith(".cram"):
    output = "/dev/stdout"
    if snakemake.params.embed_ref:
        view_options = "-O cram,embed_ref"
    else:
        view_options = "-O cram"
    convert = f" | samtools view -@ {snakemake.threads} {view_options} --reference {snakemake.input.ref} -o {snakemake.output.bam}"
else:
    output = snakemake.output.bam
    convert = ""

with tempfile.TemporaryDirectory() as tmpdir:
    shell(
        "(picard MarkDuplicates"  # Tool and its subcommand
        " {java_opts}"  # Automatic java option
        " {extra}"  # User defined parmeters
        " {bams}"  # Input bam(s)
        " --TMP_DIR {tmpdir}"
        " --OUTPUT {output}"  # Output bam
        " --METRICS_FILE {snakemake.output.metrics}"  # Output metrics
        " {convert} ) {log}"  # Logging
    )

__author__ = "Johannes Köster, Jorge Langa"
__copyright__ = "Copyright 2016, Johannes Köster"
__email__ = "[email protected]"
__license__ = "MIT"


from snakemake.shell import shell
from snakemake_wrapper_utils.java import get_java_opts

# Distribute available threads between trimmomatic itself and any potential pigz instances
def distribute_threads(input_files, output_files, available_threads):
    gzipped_input_files = sum(1 for file in input_files if file.endswith(".gz"))
    gzipped_output_files = sum(1 for file in output_files if file.endswith(".gz"))
    potential_threads_per_process = available_threads // (
        1 + gzipped_input_files + gzipped_output_files
    )
    if potential_threads_per_process > 0:
        # decompressing pigz creates at most 4 threads
        pigz_input_threads = (
            min(4, potential_threads_per_process) if gzipped_input_files != 0 else 0
        )
        pigz_output_threads = (
            (available_threads - pigz_input_threads * gzipped_input_files)
            // (1 + gzipped_output_files)
            if gzipped_output_files != 0
            else 0
        )
        trimmomatic_threads = (
            available_threads
            - pigz_input_threads * gzipped_input_files
            - pigz_output_threads * gzipped_output_files
        )
    else:
        # not enough threads for pigz
        pigz_input_threads = 0
        pigz_output_threads = 0
        trimmomatic_threads = available_threads
    return trimmomatic_threads, pigz_input_threads, pigz_output_threads


def compose_input_gz(filename, threads):
    if filename.endswith(".gz") and threads > 0:
        return "<(pigz -p {threads} --decompress --stdout {filename})".format(
            threads=threads, filename=filename
        )
    return filename


def compose_output_gz(filename, threads, compression_level):
    if filename.endswith(".gz") and threads > 0:
        return ">(pigz -p {threads} {compression_level} > {filename})".format(
            threads=threads, compression_level=compression_level, filename=filename
        )
    return filename


extra = snakemake.params.get("extra", "")
java_opts = get_java_opts(snakemake)
log = snakemake.log_fmt_shell(stdout=True, stderr=True)
compression_level = snakemake.params.get("compression_level", "-5")
trimmer = " ".join(snakemake.params.trimmer)

# Distribute threads
input_files = [snakemake.input.r1, snakemake.input.r2]
output_files = [
    snakemake.output.r1,
    snakemake.output.r1_unpaired,
    snakemake.output.r2,
    snakemake.output.r2_unpaired,
]

trimmomatic_threads, input_threads, output_threads = distribute_threads(
    input_files, output_files, snakemake.threads
)

input_r1, input_r2 = [
    compose_input_gz(filename, input_threads) for filename in input_files
]

output_r1, output_r1_unp, output_r2, output_r2_unp = [
    compose_output_gz(filename, output_threads, compression_level)
    for filename in output_files
]

shell(
    "trimmomatic PE -threads {trimmomatic_threads} {java_opts} {extra} "
    "{input_r1} {input_r2} "
    "{output_r1} {output_r1_unp} "
    "{output_r2} {output_r2_unp} "
    "{trimmer} "
    "{log}"
)