Keywords and Expertise

RNA sequences and structures.

DNA sequences and structure, including processes such as methylation and replication.|The DNA sequences might be coding or non-coding sequences.

Whole genomes of one or more organisms, or genomes in general, such as meta-information on genomes, genome projects, gene names etc.

A topic concerning high-throughput sequencing of cDNA to measure the RNA content (transcriptome) of a sample, for example, to investigate how different alleles of a gene are expressed, detect post-transcriptional mutations or identify gene fusions.|This includes small RNA profiling (small RNA-Seq), for example to find novel small RNAs, characterize mutations and analyze expression of small RNAs.

Metagenomics is the study of genetic material recovered directly from environmental samples, without the need for culturing individual organisms.

The analysis of protein-DNA interactions where chromatin immunoprecipitation (ChIP) is used in combination with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.

ATAC-seq or Assay for Transposase-Accessible Chromatin using sequencing is a popular NGS technique used to profile open chromatin regions. It provides insights into regions of the genome that are accessible to DNA-binding proteins, such as transcription factors, and can help identify regulatory elements like enhancers and promoters

The study of genes, genetic variation and heredity in living organisms.

The archival, processing and analysis of molecular sequences (monomer composition of polymers) including molecular sequence data resources, sequence sites, alignments, motifs and profiles.

Preeclampsia is persistent high blood pressure that develops during pregnancy or the postpartum period and is often associated with high levels of protein in the urine OR the new development of decreased blood platelets, trouble with the kidneys or liver, fluid in the lungs, or signs of brain trouble such as seizures and/or visual disturbances.

Nanopore is a technology that allows for the sequencing of DNA and RNA by passing a strand of nucleic acid through a tiny pore and measuring changes in electrical current as the strand passes through.

Protein and peptide identification, especially in the study of whole proteomes of organisms.|Includes metaproteomics: proteomics analysis of an environmental sample.|Proteomics includes any methods (especially high-throughput) that separate, characterize and identify expressed proteins such as mass spectrometry, two-dimensional gel electrophoresis and protein microarrays, as well as in-silico methods that perform proteolytic or mass calculations on a protein sequence and other analyses of protein production data, for example in different cells or tissues.

Restriction-site associated DNA sequencing.

study, understand and explore the genomics of viruses to gain insights into their evolution, pathogenesis, and potential treatments

The study of evolutionary relationships amongst organisms.|This includes diverse phylogenetic methods, including phylogenetic tree construction, typically from molecular sequence or morphological data, methods that simulate DNA sequence evolution, a phylogenetic tree or the underlying data, or which estimate or use molecular clock and stratigraphic (age) data, methods for studying gene evolution etc.

Organism classification, identification and naming.

The application of information technology to chemistry in biological research environment.

The exploitation of biological process, structure and function for industrial purposes, for example the genetic manipulation of microorganisms for the antibody production.

The study of life and living organisms, including their morphology, biochemistry, physiology, development, evolution, and so on.

Objective indicators of biological state often used to assess health, and determinate treatment.

The degree of variation of life forms within a given ecosystem, biome or an entire planet.

The assembly of fragments of a DNA sequence to reconstruct the original sequence.|Assembly has two broad types, de-novo and re-sequencing. Re-sequencing is a specialised case of assembly, where an assembled (typically de-novo assembled) reference genome is available and is about 95% identical to the re-sequenced genome. All other cases of assembly are 'de-novo'.

Laboratory technique to sequence the complete DNA sequence of an organism's genome at a single time.

Protein-protein, protein-DNA/RNA and protein-ligand interactions, including analysis of known interactions and prediction of putative interactions.|This includes experimental (e.g. yeast two-hybrid) and computational analysis techniques.

An experiment for studying protein-protein interactions.|This used to have the ID http://edamontology.org/topic_3557 but the numerical part (owing to an error) duplicated http://edamontology.org/operation_3557 ('Imputation'). ID of this concept set to http://edamontology.org/topic_3957 in EDAM 1.24.

The analysis of levels and patterns of synthesis of gene products (proteins and functional RNA) including interpretation in functional terms of gene expression data.|Gene expression levels are analysed by identifying, quantifying or comparing mRNA transcripts, for example using microarrays, RNA-seq, northern blots, gene-indexed expression profiles etc.|This includes the study of codon usage in nucleotide sequence(s), genetic codes and so on.

The study of gene and protein function including the prediction of functional properties of a protein.

The processes that need to be in place to ensure the quality of products for human or animal use.

Stable, naturally occuring mutations in a nucleotide sequence including alleles, naturally occurring mutations such as single base nucleotide substitutions, deletions and insertions, RFLPs and other polymorphisms.

The quality, integrity, and cleaning up of data.

Protein secondary or tertiary structural data and/or associated annotation.

Large-scale study (typically comparison) of DNA sequences of populations.

Explore methods to mitigate the risk of COVID-10 outbreaks

The acquisition of data, typically measurements of physical systems using any type of sampling system, or by another other means.

Topic concerning the identity of biological entities, or reports on such entities, and the mapping of entities and records in different databases.

Rendering (drawing on a computer screen) or visualisation of molecular sequences, structures or other biomolecular data.

DNA mutation.

The study (typically comparison) of the sequence, structure or function of multiple genomes.

Approach which samples, in parallel, all genes in all organisms present in a given sample, e.g. to provide insight into biodiversity and function.

The curation, processing, analysis and prediction of data about the structure of biological molecules, typically proteins and nucleic acids and other macromolecules.|This includes related concepts such as structural properties, alignments and structural motifs.

The translation of mRNA into protein and subsequent protein processing in the cell.

Plants, e.g. information on a specific plant genome including molecular sequences, genes and annotation.|The resource may be specific to a plant, a group of plants or all plants.

Genome-wide association study experiments.

The use of imaging techniques for clinical purposes for medical research.

RNA splicing; post-transcription RNA modification involving the removal of introns and joining of exons.|This includes the study of splice sites, splicing patterns, alternative splicing events and variants, isoforms, etc..

The common steps involved in scRNAseq data processing are quality control, read alignment, gene expression quantification, normalization, dimensionality reduction, clustering, and differential gene expression analysis.

Multi-omics data analysis refers to the integration and analysis of multiple types of biological data, such as genomics, transcriptomics, proteomics, and metabolomics, to gain a more comprehensive understanding of biological systems.

The study and simulation of molecular conformations using a computational model and computer simulations.|This includes methods such as Molecular Dynamics, Coarse-grained dynamics, metadynamics, Quantum Mechanics, QM/MM, Markov State Models, etc.